1.2.1 Safety and rules in biology laboratory (Q&A)

Short Answer Questions

1. Why is maintaining safety in the biology lab important?
   Answer
   It prevents accidents and ensures the well-being of everyone in the lab.
2. What is the purpose of wearing goggles in the laboratory?
   Answer
   Goggles protect the eyes from chemical splashes, projectiles, and harmful substances.
3. Why should face masks be used in a biology lab?
   Answer
   Face masks protect the respiratory system from inhaling hazardous vapors, fumes, and dust.
4. What is the purpose of a lab coat?
   Answer
   Lab coats protect clothing and skin from chemical and biological spills.
5. What type of shoes should be worn in a biology lab?
   Answer
   Closed shoes should be worn to protect feet from chemical spills, broken glass, and falling objects.
6. What is an emergency eyewash station used for?
   Answer
   It washes the eyes with saline in case of chemical exposure.
7. What is the purpose of a laminar flow cabinet?
   Answer
   It provides a sterile environment for experiments that require contamination-free settings.
8. When should a safety shower be used?
   Answer
   A safety shower is used to wash off chemicals that have splashed onto the body.
9. What should be done in case of a chemical spill?
   Answer
   Notify the teacher, cordon off the area, wear appropriate PPE, and use absorbents to clean the spill.
10. What kind of waste should not be disposed of in sinks?
    Answer
    Toxic materials, heavy metals, organic solvents, and biohazardous waste should not be disposed of in sinks.
11. What is Category A waste?
    Answer
    Category A waste refers to sharp waste like needles, syringes, glass, and scalpels.
12. What does Category D waste include?
    Answer
    Category D waste includes liquids such as broth cultures, liquid media, blood, and blood products.
13. What are two main purposes of a fume hood?
    Answer
    It contains toxic vapors and gases and prevents inhalation of harmful substances.
14. Why is it important to tie back long hair in the lab?
    Answer
    To avoid accidents and prevent contamination.
15. What is the first thing to do in case of a fire in the lab?
    Answer
    Stop all work immediately and turn off electrical sources if it is safe to do so.
16. How should a minor cut be treated in the lab?
    Answer
    Clean the wound with water, disinfect it with an antiseptic, and cover it with a plaster.
17. What is the purpose of a biological safety cabinet?
    Answer
    It prevents contamination when working with microorganisms.
18. Why should unused chemicals not be returned to stock bottles?
    Answer
    To prevent contamination of stock reagents.
19. What should be done after every experiment?
    Answer
    Clean the work area to prevent cross-contamination and ensure a tidy environment.
20. Why is it essential to behave responsibly in the lab?
    Answer
    To ensure safety for yourself and others in the laboratory.

Explanation Questions

1. Why is wearing personal protective equipment (PPE) necessary in the lab?
   Answer
   PPE minimizes exposure to hazardous materials and protects the body from injury or contamination.
2. How does a fume hood protect laboratory personnel?
   Answer
   It contains toxic vapors, preventing inhalation and reducing the risk of laboratory pollution.
3. Explain the importance of knowing the location of safety equipment in the lab.
   Answer
   Knowing the location helps respond quickly in emergencies, minimizing injury or damage.
4. What steps should be taken in case of an electrical shock?
   Answer
   Turn off the power supply, avoid touching the person, and seek immediate medical help.
5. Describe the purpose of a laminar flow cabinet in biological research.
   Answer
   It provides a sterile environment, protecting samples from contamination.
6. Why is waste management crucial in the biology laboratory?
   Answer
   Proper waste management reduces risks to health and the environment.
7. How can chemical spills be safely managed?
   Answer
   Notify the teacher, use appropriate absorbents, and wear PPE before cleaning the spill.
8. What are the risks of not wearing closed shoes in the laboratory?
   Answer
   Feet are exposed to chemical spills, broken glass, and falling objects, increasing injury risk.
9. Why is it essential to handle chemicals with care?
   Answer
   Improper handling can lead to burns, inhalation of toxic fumes, or accidental ingestion.
10. How does proper disposal of biological waste benefit the environment?
    Answer
    It prevents environmental pollution and reduces health risks.